Gene therapy of cystic fibrosis: The glycofection approach
Identifieur interne : 000381 ( France/Analysis ); précédent : 000380; suivant : 000382Gene therapy of cystic fibrosis: The glycofection approach
Auteurs : Isabelle Fajac [France] ; Pascale Briand [France] ; Michel Monsigny [France]Source :
- Glycoconjugate Journal [ 0282-0080 ] ; 2001-09-01.
English descriptors
Abstract
Abstract: Many cells express surface membrane lectins that selectively bind and carry glycoconjugates into intracellular endosomes; in addition, various intracellular membrane and soluble lectins act as shuttles between different compartments. On this basis, we developed glycosylated polycations, now called glycofectins (glycosylated polylysine and polyethyleneimine). Recently, we set up a simple way to transform oligosaccharides into glycosynthons suitable to substitute proteins or polymers. Glycofectins bind plasmid DNA leading to compact glycoplexes. Glycoplexes prepared with glycofectins were found to be much more active than naked plasmid to transfer genes to various types of cells including human airway epithelial and serous cells. The gene transfer efficiency was found to depend on the nature of the sugars borne by glycofectins. It appeared that the sugar-dependent efficiency was not only related to the uptake but also to the intracellular traffic of glycoplexes.
Url:
DOI: 10.1023/A:1020827507748
Affiliations:
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ISTEX:825D16FEA59D0B1C9D45B4CF1DB4E48D436141B2Le document en format XML
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